FastQCFastQC Report
Tue 21 Aug 2018
3701_S16_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3701_S16_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82698860
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4018490.48591842741242136No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2357090.2850208576031157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2640000.080.3440259
CACCCG3446000.067.6718
GCACCC4016250.055.740987
GGCACC4417800.047.9213456
TGGCAC4669350.043.3427285
TTGGCA5758500.033.8183944
CCTTGG6176550.026.0359942
CTTGGC7477100.024.9728763
GCCTTG4413050.019.8387721
ACCCGG1358500.019.812839
CGAGAA2630050.017.444110-14
AACCGA802900.014.2761879
CCGAGA3095300.014.145310-14
CAACCG694000.012.9523568
CCCGAG3448250.012.18706410-14
GAGAAT4057850.011.35703110-14
ACCTTG3277200.011.1654581
AATTCC4680050.010.82719315-19
AACCCG739300.010.7276938
AGAATT4582750.010.36586510-14
Produced by FastQC (version 0.11.3)