FastQCFastQC Report
Tue 21 Aug 2018
60_S1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename60_S1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63812749
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACCTGTACACTGTTATCTTCATTAATATTTTATTATCCCTAACGCT735100.11519641631486523No Hit
AAGTACCGTTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTT714830.11201993507598301No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC656100.10281644503357784RNA PCR Primer, Index 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG903050.037.3022881
CGACGA1081100.031.1925982
GACGAT1386950.024.3617973
TCCGAC794550.022.0981461
CGATCA682750.016.9486775
CGATCC559200.014.8610985
GTACAC1129350.010.7372839
ACACGT679250.010.537433
ACGTTA227650.010.2259681
CACGTC730200.09.9920014
TACCGT1236050.09.1670534
CGTTAA1241200.09.081947
ACGTCT852250.08.8521315
TAACGC285850.08.75021840-44
ACCGTT1373700.08.4396875
CCGTTA1347600.08.4136486
CACACG916350.08.16288952
GTACCG1458900.07.89181383
CACCCG1091150.07.37539636
CTAACG355900.07.16753140-44
Produced by FastQC (version 0.11.3)