FastQCFastQC Report
Tue 21 Aug 2018
60_S1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename60_S1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63812749
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7744751.2136681339335498No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG814790.12768451645924234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2163800.087.9907769
CACCCG2857350.073.887678
GCACCC3447600.058.8271757
TGGCAC3757300.049.0653275
GGCACC4453700.043.372256
TTGGCA4408000.040.32944
CTTGGC5084950.033.5599983
CCTTGG5378500.027.9128722
GCCTTG3540250.023.0848791
ACCCGG1078750.023.0367269
CGAGAA2272550.018.07699210-14
AACCGA550250.017.1581769
CAACCG479250.016.2442348
CCGAGA2513050.015.63447910-14
CCCGAG2754550.013.5658910-14
ACCTTG2588100.013.4475161
GAGAAT3502000.011.80671710-14
ATTCCA4073600.011.78813615-19
AATTCC3800750.011.75214115-19
AACCCG514750.011.5520278
Produced by FastQC (version 0.11.3)