FastQCFastQC Report
Tue 21 Aug 2018
61_S2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename61_S2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73402774
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC959510.1307184930095421RNA PCR Primer, Index 2 (100% over 50bp)
AAGTACCGTTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTT911410.12416560714721764No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG924850.035.0265851
ACGATC1028600.031.2177454
CGACGA1201350.027.1532552
GACGAT1565550.021.0188063
TCCGAC908450.017.0863951
CGATCA704900.015.5674335
CGATCT632550.014.1644665
ACGTTA256150.013.6181311
CGATCC612600.013.4466355
CGATCG667300.011.1088545
GTACAC1224150.07.7481929
TAATCG559950.07.5183625
CGTTAA1862150.07.42482477
TACCGT1904800.07.4042174
CCGTTA1957900.07.07670556
ACCGTT2018950.06.97835975
TAACGC302100.06.591723440-44
GTACCG2196600.06.54355433
TTAAGC2369300.06.05109749
GTTAAG2519600.05.8349238
Produced by FastQC (version 0.11.3)