FastQCFastQC Report
Tue 21 Aug 2018
61_S2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename61_S2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73402774
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7814911.0646613982190918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA1766400.072.8670359
CACCCG2504200.056.8207638
GCACCC3253250.042.2958877
TGGCAC3658400.033.9322135
TTGGCA4266350.028.028054
GGCACC4842750.026.9309046
CTTGGC5155050.022.2648813
CCTTGG6229250.016.414672
CGAGAA1860650.015.22192110-14
GCCTTG3891900.014.1450141
ACCCGG1230650.013.8198939
CCGAGA2139650.012.55879410-14
AACCGA560650.011.5692959
AGCGTA459100.011.5378879
CAACCG505150.010.5582998
TAATCG476150.010.5577745
CCCGAG2484550.010.38557210-14
TAGCGT533400.09.9854318
AATCGC554650.09.4055456
GAGAAT3130900.09.23354610-14
Produced by FastQC (version 0.11.3)