FastQCFastQC Report
Tue 21 Aug 2018
62_S3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename62_S3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105318836
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC1906380.1810103560202659RNA PCR Primer, Index 3 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG1421800.038.0272941
ACGATC1502200.035.3926664
CGACGA1746700.030.9748082
TCCGAC1274100.024.6514841
GACGAT2279250.023.9892023
CGATCA977950.019.5617775
TAGGTT897450.018.3895859
CGATCT865950.016.8005925
GTAGTA1039250.016.2736765
CGATCC821200.015.708175
GGTAGT1158300.014.9601114
GTAGGT1229650.014.43611058
TAGTAG1315700.013.3977656
CGATCG962350.011.8348335
ACGTTA320400.010.9098141
AGTAGG1806550.09.955357
AGGTAG1976100.09.3597273
GAGGTA1909200.09.3378382
TAGGTA835400.09.2402455
GGTAAG1037350.07.93365577
Produced by FastQC (version 0.11.3)