FastQCFastQC Report
Tue 21 Aug 2018
62_S3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename62_S3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105318836
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1125610.10687641857340695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2959350.073.9593059
CACCCG4049900.060.5113268
GCACCC4946150.047.767587
TGGCAC5427500.039.5520675
TTGGCA6221150.033.2302864
GGCACC6828700.032.8498426
CTTGGC7467200.026.712943
CCTTGG8599450.020.6584472
GCCTTG5614250.018.0788861
ACCCGG1728150.017.7998359
CGAGAA2989200.015.85146710-14
AACCGA814300.013.4311759
CCGAGA3441350.013.20381710-14
CAACCG731650.012.6248778
AACCTA959050.011.5865869
CCCGAG3851550.011.32772410-14
AATTCC4995950.010.5146215-19
ACCTTG4005700.010.4717431
GAGAAT4578050.010.45426110-14
ATTCCA5559800.010.27423915-19
Produced by FastQC (version 0.11.3)