FastQCFastQC Report
Tue 21 Aug 2018
63_S4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63_S4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58522441
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG740250.036.8300861
ACGATC810700.033.0023234
CGACGA946900.028.9002382
GACGAT1224200.022.4391843
TCCGAC757850.017.7755361
CGATCA571100.016.1355935
CGATCT508350.015.3285885
CGATCC500100.013.3201065
CGATCG554900.011.4261215
ACGTTA205600.010.68361
GTACAC921550.06.887989
CGTTAA761750.06.50352537
TACCGT805000.06.3988594
TAATCG428650.06.3148765
CCGTTA805500.06.18655546
ACCGTT883400.05.8722695
TAACGC235000.05.856508740-44
TAGGTT519550.05.71555669
GTAGGT662600.05.71488438
GGGGGG166905450.05.5912714145-146
Produced by FastQC (version 0.11.3)