FastQCFastQC Report
Tue 21 Aug 2018
63_S4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63_S4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58522441
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170190.5417050187636568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA1570850.074.566929
CACCCG2172600.059.9420748
GCACCC2797600.044.563357
TGGCAC3121200.036.310435
TTGGCA3483250.031.1475564
GGCACC4057000.029.25686
CTTGGC4168550.025.0870573
CCTTGG5328850.017.5040422
GCCTTG3286450.015.9649411
ACCCGG1055250.015.6261049
CGAGAA1631850.015.57201610-14
CCGAGA1855050.013.01070910-14
AACCGA461700.011.8522029
CCCGAG2143350.010.86886910-14
TAATCG433900.010.8627685
CAACCG426900.010.4256078
GAGAAT2569700.09.9855610-14
AATCGC492200.09.8577476
AATTCC2892600.09.72138615-19
ATTCCA3206450.09.57547615-19
Produced by FastQC (version 0.11.3)