FastQCFastQC Report
Thu 23 Aug 2018
64_S27_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename64_S27_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71836871
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC2073920.2886985431200087RNA PCR Primer, Index 5 (100% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCGCGTATGC898480.12507226268248794RNA PCR Primer, Index 5 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG876400.033.3753431
ACGATC875200.032.495294
ACGTTA234500.025.5258221
CGACGA1154750.025.336522
GACGAT1460550.020.1767443
TCCGAC759800.019.1188831
TAGGTT584500.018.8825749
GTAGTA641000.017.3326665
CGATCA634400.017.1677915
GGTAGT754200.016.7249954
CGATCT518700.014.8612865
GTAGGT839050.014.3198178
TAGTAG856500.013.5938376
CGATCC502550.013.0583755
ATAGGC576950.011.9184973
CGTTGA456550.011.911781
GGGTCG751950.011.3774752
CGGGTT538450.011.3604128
CGATCG637900.010.8140395
TAATCG609500.010.7550245
Produced by FastQC (version 0.11.3)