FastQCFastQC Report
Thu 23 Aug 2018
64_S27_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename64_S27_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71836871
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA1526550.055.7212269
CACCCG2264500.041.8178868
GCACCC2772650.034.190687
TGGCAC2973950.029.2893645
GGCACC3788800.024.2809816
TTGGCA3512400.023.6479844
CTTGGC4589000.017.5512833
CCTTGG4865050.015.1930362
GCCTTG3371150.013.90566351
CGAGAA1584150.011.61637610-14
CGACCC734400.011.5396735
ACCCGG1083550.010.6776169
CCCCCG1095050.010.2721814
CCGACC910350.010.0470064
AACCTA722300.09.8431779
CCGAGA1822200.09.83443710-14
CCCCCC1615900.09.7980363
TAATCG408650.09.6993435
GTTGCG520250.09.4286852
CCCGAC1055150.08.7235813
Produced by FastQC (version 0.11.3)