FastQCFastQC Report
Tue 21 Aug 2018
65_S6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename65_S6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72308400
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC1040010.14382976251721794RNA PCR Primer, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG1002300.035.078681
ACGATC1034100.033.55614
CGACGA1238750.028.595012
TCCGAC851250.023.4034391
GACGAT1612250.022.1113073
CGATCA734450.017.2953245
CGATCT589400.015.69644455
CGATCC596450.014.2264925
ACGTTA249450.012.7841571
TAGGTT642700.012.180959
CGATCG599650.011.9726275
GTAGTA756750.010.6248145
GGTAGT806000.010.2743184
GTAGGT828100.010.2203318
GTACAC1146450.09.2342599
TAGTAG993200.08.8079456
AGTAGG1269300.06.97821247
TAACGC319900.06.80498740-44
AGGTAG1451850.06.41242463
GAGGTA1356150.06.40882162
Produced by FastQC (version 0.11.3)