FastQCFastQC Report
Tue 21 Aug 2018
65_S6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename65_S6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72308400
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1684470.23295633702308444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA1575650.063.851199
CACCCG2318900.047.9794548
GCACCC2965850.036.059657
TGGCAC3364650.028.6109665
GGCACC3974600.025.3730936
TTGGCA4149950.022.4637364
CTTGGC5291050.017.1143673
CCTTGG5589050.014.2087472
CGAGAA1631500.013.49619210-14
ACCCGG1136500.011.9602259
GCCTTG3761250.011.6528111
CCGAGA1934700.010.90245510-14
AACCGA546100.09.6062239
CCCGAG2268850.09.03498210-14
CAACCG498250.08.7129588
GTTGCG640500.08.6462532
TAATCG402200.08.4354235
AACCTA674600.08.0684199
AATTCC3079350.08.06240515-19
ATTCCA3464850.07.846832315-19
Produced by FastQC (version 0.11.3)