FastQCFastQC Report
Tue 21 Aug 2018
66_S7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename66_S7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83736171
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC1201310.1434636890669386RNA PCR Primer, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG1153700.039.0904661
ACGATC1201650.036.6126634
CGACGA1419500.031.7913762
GACGAT1827300.024.72773
TCCGAC1016600.024.528451
CGATCA769800.019.3823935
CGATCT698900.017.68445
CGATCC722600.014.7214835
TAGGTT704550.013.0067039
CGATCG793700.012.2996215
GTAGTA775600.011.6741695
GGTAGT854500.011.2451624
GTAGGT895600.010.8512458
TAGTAG973650.09.7341566
ACGTTA260300.08.7469311
GAGGTA1422200.06.88600162
AGGTAG1520250.06.82939773
AGTAGG1495050.06.6858137
GGTCGG1000100.06.6461093
CGTTAA1443100.06.56753067
Produced by FastQC (version 0.11.3)