FastQCFastQC Report
Tue 21 Aug 2018
66_S7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename66_S7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83736171
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2245250.073.278829
CACCCG3055500.060.341988
GCACCC3797550.046.718117
TGGCAC4250700.038.1021965
GGCACC5361050.031.628816
TTGGCA5047050.030.8672224
CTTGGC6035850.024.8192963
CCTTGG6558550.020.3275592
ACCCGG1309650.018.3456489
GCCTTG4455650.016.7898521
CGAGAA2293600.015.69540210-14
AACCGA642400.013.3680339
CCGAGA2669100.012.87013810-14
CAACCG575650.012.02838
CCCGAG2996850.010.97833810-14
ACCTTG3103450.010.5068511
GAGAAT3468450.010.39332210-14
AATTCC3886200.010.25722215-19
AGAATT3721250.010.00176810-14
ATTCCA4374550.09.90941915-19
Produced by FastQC (version 0.11.3)