FastQCFastQC Report
Tue 21 Aug 2018
67_S8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename67_S8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81692564
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACCGTTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTT846660.1036397878269557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG1151550.039.4745181
ACGATC1220700.036.6152344
CGACGA1400650.032.4853942
TCCGAC1023300.024.910181
GACGAT1861400.024.5570373
CGATCA775500.019.4564045
CGATCT708350.017.8090575
CGATCC711750.015.1099785
CGATCG776100.012.8042225
ACGTTA264400.010.2674541
TAGGTT670200.09.3623459
GTAGTA772350.08.3603215
GTAGGT851400.08.22674758
GGTAGT831050.08.2263334
CCGTTA1803700.07.26500376
TAGTAG951050.07.2420666
CGTTAA1829450.07.15072977
TACCGT1865500.07.1142854
ACCGTT1929550.06.9424145
GTACCG2085550.06.47910453
Produced by FastQC (version 0.11.3)