FastQCFastQC Report
Tue 21 Aug 2018
67_S8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename67_S8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81692564
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2247450.077.9825449
CACCCG3019400.064.6517948
GCACCC3698450.050.6035357
TGGCAC4188950.040.3760035
GGCACC5309300.033.528436
TTGGCA4966350.032.746814
CTTGGC5874350.026.5859223
CCTTGG6304550.022.0110532
ACCCGG1248800.019.2921459
GCCTTG4335650.017.7947771
CGAGAA2314050.016.50111210-14
AACCGA620900.013.9131789
CCGAGA2698800.013.51226310-14
CAACCG548700.012.9648048
CCCGAG2973600.011.73172310-14
ACCTTG2982100.011.2573841
GAGAAT3532400.010.8466210-14
AATTCC3933700.010.6815615-19
AGAATT3798450.010.42313410-14
ATTCCA4443700.010.29704615-19
Produced by FastQC (version 0.11.3)