FastQCFastQC Report
Tue 21 Aug 2018
68_S9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename68_S9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78590475
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC1142920.1454272925567634RNA PCR Primer, Index 9 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG1056950.036.1714671
CGACGA1306900.029.348452
GACGAT1671400.022.9876563
TCCGAC901750.022.6429861
TAGGTT686050.018.4409799
GTAGTA805900.016.4228765
CGATCT637450.016.1500555
GGTAGT848650.015.8277184
GTAGGT902150.015.3014668
CGATCC649700.013.5658555
TAGTAG1042450.013.1231816
ACGTTA244150.012.9719471
CGATCG744700.011.3258195
AGTAGG1388650.010.1193857
AGGTAG1498400.09.660693
GAGGTA1439100.09.4571452
GTACAC1138500.08.1516349
CGTTAA907500.07.37247567
GGTCGG934950.06.9843963
TAACGC263700.06.96435140-44
Produced by FastQC (version 0.11.3)