FastQCFastQC Report
Tue 21 Aug 2018
68_S9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename68_S9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78590475
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT799770.10176424051387906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2178400.076.448549
CACCCG3014250.061.5670668
GCACCC3690150.048.3426937
TGGCAC4038350.039.9810185
GGCACC4962700.034.1484576
TTGGCA4766950.032.6028374
CTTGGC5910100.025.3695643
CCTTGG6475400.020.6695582
ACCCGG1272700.017.8635359
GCCTTG4246850.017.0637111
CGAGAA2230250.016.2198810-14
AACCGA580100.014.6150169
CCGAGA2539600.013.64272510-14
CAACCG527050.013.1512748
GTTGCG712300.012.9270732
AACCTA733250.011.6122079
CCCGAG2896250.011.47226210-14
TAATCG458650.010.89695555
ACCTTG3132550.010.6886731
AATTCC3774400.010.63766515-19
Produced by FastQC (version 0.11.3)