FastQCFastQC Report
Tue 21 Aug 2018
69_S10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename69_S10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80929987
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG1186200.036.8934361
ACGATC1224350.034.8134734
CGACGA1432700.030.3930132
GACGAT1792350.024.4527423
TCCGAC990600.021.3119451
CGATCA842250.017.8190565
CGATCT706000.016.8864655
CGATCC697350.015.2126545
CGATCG777400.011.46880155
ACGTTA259100.010.8715851
GTACAC1295900.010.0554349
TAGGTT697450.08.4002719
CACCCG1489750.08.1788926
TAACGC338000.07.88468840-44
GTAGGT899950.07.5478318
GTAGTA838300.07.21516855
GGTAGT873100.07.17828274
CTAACG427350.06.338731340-44
TGTACA2276450.06.2626388
CGCTAA431250.06.20291945-49
Produced by FastQC (version 0.11.3)