FastQCFastQC Report
Tue 21 Aug 2018
69_S10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename69_S10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80929987
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1701520.2102459252835417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2955800.089.014219
CACCCG3963950.073.65138
GCACCC4724200.059.4204187
TGGCAC5003600.051.0672655
GGCACC6126950.043.5584346
TTGGCA5788050.042.497344
CTTGGC6999800.033.750053
CCTTGG7620050.027.3706282
GCCTTG4871200.023.6615431
ACCCGG1507800.022.9560729
AACCGA654250.019.8505679
CGAGAA3062150.018.58459910-14
CAACCG591150.017.587898
CCGAGA3311850.016.26812610-14
ACCTTG3505550.013.9035191
CCCGAG3740850.013.7548810-14
ATTCCA5220700.012.68350115-19
GAGAAT4493050.012.64024510-14
AATTCC4895650.012.52404215-19
AACCCG648100.012.3498368
Produced by FastQC (version 0.11.3)