FastQCFastQC Report
Tue 21 Aug 2018
70_S11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename70_S11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98161726
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC1449660.14768077733270502RNA PCR Primer, Index 11 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG1304100.036.804071
ACGATC1356900.034.811154
CGACGA1642950.029.3022542
TCCGAC1127300.023.4599761
GACGAT2116250.023.0098743
CGATCA908400.019.0515825
TAGGTT851900.018.6620549
GTAGTA967300.016.6620355
CGATCT795250.016.2391435
GGTAGT1049600.015.7934884
GTAGGT1086800.015.5146218
CGATCC792850.014.0705145
TAGTAG1228800.013.8109016
CGATCG909200.011.3871875
GAGGTA1738650.09.8845672
AGTAGG1750800.09.6889627
ACGTTA319600.09.6585631
AGGTAG1866600.09.6508343
GTACAC1382250.07.5903739
CGTTAA1371950.07.09428027
Produced by FastQC (version 0.11.3)