FastQCFastQC Report
Tue 21 Aug 2018
70_S11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename70_S11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98161726
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1824330.1858494215963562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2362700.067.894419
CACCCG3307900.053.8209888
GCACCC4160300.041.350317
TGGCAC4672800.033.4081045
GGCACC5734850.028.5774046
TTGGCA5649750.026.7349554
CTTGGC7037250.020.692383
CCTTGG7469000.017.256642
ACCCGG1535250.014.5139749
CGAGAA2429250.014.49302510-14
GCCTTG5108250.014.1991721
CCGAGA2868050.011.69976710-14
AACCTA896950.010.7700129
AACCGA741100.010.4554839
CCCGAG3249450.09.97040510-14
CAACCG652000.09.6685768
AATTCC4175300.09.31250215-19
GAGAAT3973950.09.00600910-14
ATTCCA4767000.08.9777115-19
AGAATT4241700.08.76275710-14
Produced by FastQC (version 0.11.3)