FastQCFastQC Report
Tue 21 Aug 2018
71_S12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename71_S12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66746402
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC19390292.905068950383273RNA PCR Primer, Index 12 (100% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCGCGTATGC5634180.8441174102538143RNA PCR Primer, Index 12 (98% over 50bp)
AAATGGATTTTTGGAAGTAGGAGTTGGAATTCTCGGGTGCCAAGGAACTC1305890.19564949733170636RNA PCR Primer, Index 1 (100% over 26bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATG718010.10757283965658554RNA PCR Primer, Index 12 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG970850.024.4738541
ACGATC1011300.023.541144
CGACGA1177150.020.823242
TAGGTT515900.018.3426849
GACGAT1542100.016.0863593
TCCGAC838950.015.4220741
CGATCA571300.015.376335
GATTTT1761700.015.0171066
CGTTGA577350.014.8008892
GTAGTA797150.013.8938345
GTAGGT739550.013.6539098
GGTAGT850400.013.4957144
ACGTTG664550.013.1991791
GGATTT2154150.013.026385
ACGTTA225550.012.1650851
GATCGG905450.012.0063836
CGATCC471700.011.9874345
TAGTAG979700.011.7071146
ATGGAT2509250.011.659823
GGATCG825250.011.6260086
Produced by FastQC (version 0.11.3)