FastQCFastQC Report
Tue 21 Aug 2018
71_S12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename71_S12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66746402
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA7870661.1791886549929689Illumina RNA PCR Primer (100% over 45bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940180.4405001486072612No Hit
ACTCCTACTTCCAAAAATCCATTTGATCGTCGGACTGTAGAACTCTGAAC1264570.18945890147007474Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG752000.11266524898225976No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTATATCTCGGTGGTCGCCGTA693030.103830315827361Illumina RNA PCR Primer (97% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGGA673800.10094926165458327Illumina RNA PCR Primer (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2523200.051.09289
CACCCG3257900.043.54598
GCACCC3361500.040.074547
TGGCAC3403800.035.913185
GGCACC3680250.034.7856566
TTGGCA3848650.030.5165824
CTTGGC4809150.023.5796853
CCTTGG4475900.021.9320342
GCCTTG2766350.019.5943621
CCTACT1148200.019.400314
TCCTAC1396950.016.4994013
CTACTT1352800.016.0562695
CTCCTA1402750.015.6876872
CGAGAA2102450.012.94461210-14
ACCCGG1272200.012.8868589
CGATCC818700.012.7887049
AACCTA553350.012.579059
TACTTC1908850.011.8721416
AACCGA569150.011.0632699
CCGAGA2463150.010.83700910-14
Produced by FastQC (version 0.11.3)