FastQCFastQC Report
Tue 21 Aug 2018
MPS1-1_S23_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMPS1-1_S23_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80868956
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGC1315020.1626112250045617RNA PCR Primer, Index 47 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG1276800.030.4011041
ACGATC1368600.027.8017184
CGACGA1618950.023.9670962
TCCGAC1097250.017.7444461
GACGAT2498350.015.9658223
CGATCA850300.015.908585
CGATCC774900.012.6922355
ACGTTA294400.011.4769221
CGATCT1253200.09.2278975
CGATCG999000.07.69053845
CGTTAA470600.07.4434032
CACCCG1384900.06.14817676
GGGGGG225783800.05.8167734145-146
TAATCG721700.05.7119665
ATTAAC1262400.05.2662761
GTACAC1298450.05.1133599
TTAACC1218700.05.09583042
Produced by FastQC (version 0.11.3)