FastQCFastQC Report
Tue 21 Aug 2018
MPS1-1_S23_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMPS1-1_S23_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80868956
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2170080.26834524734064824No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2169720.2683007308762586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2383750.076.113229
CACCCG3216900.062.2703448
GCACCC3687950.052.045567
GGCACC4145000.043.8864026
TGGCAC4346000.040.082155
TTGGCA5513350.030.4415194
CCTTGG6078600.023.04772
CTTGGC7527850.021.5701943
GCCTTG4265600.017.8492371
ACCCGG1350150.017.7035069
CGAGAA2377000.016.4991810-14
CCGAGA2919750.012.8263910-14
AACCGA754650.011.5739
CCCGAG3177700.011.31884510-14
CAACCG636750.010.897058
GAGAAT3621650.010.87537110-14
AATTCC4175950.010.40514115-19
ACCTTG3217050.010.1698631
AGAATT4109550.09.93307610-14
ATTCCA4822000.09.77675715-19
Produced by FastQC (version 0.11.3)