FastQCFastQC Report
Tue 21 Aug 2018
MPS1-2_S24_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMPS1-2_S24_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98300242
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGC2483400.2526341695069276RNA PCR Primer, Index 48 (100% over 50bp)
AAGGGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAA1132580.1152163999759024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATC1941850.022.2470344
CCGACG2036250.021.291461
CGATCA1016100.018.9206895
CGACGA2416100.018.4968262
CACCCG1904550.015.4193576
GACGAT2935350.015.0255553
ACGTTA269250.014.7712671
TCCGAC1788000.014.4481651
GGCACC3287250.011.6192134
TAGGTA1305600.011.6069775
CTTGGC3781600.010.9513391
TTGGCA3788600.010.5920972
CGATCC890000.010.1408055
TGGCAC3881100.09.9823723
GTAGGT1581150.09.9072194
GCACCC3186900.09.6956695
CGATCT1204700.09.5812095
ATTAGT1323700.09.2380123
TTAGTC957700.08.8449534
TGTAGG2435750.08.7287273
Produced by FastQC (version 0.11.3)