FastQCFastQC Report
Tue 21 Aug 2018
MPS1-2_S24_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMPS1-2_S24_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98300242
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7481310.7610673023572008No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3116170.3170053233439649No Hit
CTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC2783330.2831457932728182No Hit
GACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTT1593910.16214710844760688No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1086880.11056737785040244Illumina RNA PCR Primer (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACC5762550.064.43526
GCACCC6050650.064.1008157
TGGCAC5967650.059.8304185
ACCCGA6167300.058.3123669
CACCCG7691850.052.3208278
TTGGCA7268250.047.37354
CCTTGG7991000.036.629642
GCCTTG4797300.036.611761
CTTGGC9982000.033.390623
CGAGAA3943050.019.24002510-14
AACCGA925700.017.56079
CAACCG812800.016.1130478
CCGAGA4517800.016.06567610-14
ACCCGG3113300.015.868249
ACCTTG3559750.015.7784281
TTCGGC3120550.015.361712
GAGAAT5177850.014.41155510-14
AATTCC6242100.013.35094415-19
CTTCGG3730450.012.9831881
ATTCCA6803250.012.89723615-19
Produced by FastQC (version 0.11.3)