FastQCFastQC Report
Tue 21 Aug 2018
MPS1-3_S25_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMPS1-3_S25_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87503964
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACCTATCTCGTATGC956920.10935733151471858RNA PCR Primer, Index 29 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATC1468700.025.4546994
CCGACG1522200.024.9434471
CGACGA1840450.020.9514052
GACGAT2485100.015.7249723
CGATCA877350.015.6425625
TCCGAC1292650.014.6949311
TCGCGA360150.014.464616
CGATCC806550.011.5156655
ACGTTA298000.010.8727911
CGATCT1235100.09.0618155
TAGGTA928850.08.9704135
CGCGAA605150.08.57226857
CGCGAC975300.07.98363261
GTAGGT1130050.07.67027144
ACTCGC720300.07.52605444
GTAAGG1605650.06.6660488
CGATCG1163150.06.60517935
GGTAAG1585050.06.28317177
CTCGCG926000.06.1693975
AACTCG951950.06.12386753
Produced by FastQC (version 0.11.3)