FastQCFastQC Report
Tue 21 Aug 2018
MPS1-3_S25_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMPS1-3_S25_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87503964
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2179920.24912242832793263No Hit
CTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC963690.11013101075055297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2968800.054.2152149
GCACCC3740150.045.7554667
CACCCG4051100.044.0678758
GGCACC4001300.040.5318036
TGGCAC4269750.036.278075
TTGGCA5341800.028.0358244
CCTTGG6380150.019.8152372
CTTGGC7579800.019.1660213
GCCTTG4217200.016.4848251
CGAGAA2290500.015.2567610-14
CCGAGA2867450.011.70792710-14
AACCGA788950.011.6802069
ACCCGG1897950.011.282889
CAACCG701500.010.5360638
TTCGGC1752400.010.0052372
GAGAAT3646400.09.65380310-14
CCCGAG3386700.09.5348910-14
AATTCC4240950.09.273808515-19
ACCTTG3297850.09.0224221
AGAATT4266350.08.61927410-14
Produced by FastQC (version 0.11.3)