FastQCFastQC Report
Tue 21 Aug 2018
MPS1-4_S26_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMPS1-4_S26_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70196635
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACCTCAATCTCGTATGC922190.13137239413256774RNA PCR Primer, Index 8 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG1116400.025.6499771
ACGATC1134000.025.0347024
CGACGA1353900.021.328352
CGATCA735500.014.7609575
TCCGAC969250.014.6666511
GACGAT2042350.014.6398823
CGATCC641350.011.0690935
ACGTTA249800.09.3483581
TAGGTA579600.08.1068435
CGATCT1087750.08.0490845
GTAGGT741600.06.64092064
CGATCG917150.06.50739535
CGCGAC533500.05.96389341
GGGGGG192264400.05.7135377145-146
GTAAGG1099150.05.5626058
GGTAAG1035250.05.46899657
ATTAAC1088600.05.0746411
Produced by FastQC (version 0.11.3)