FastQCFastQC Report
Tue 21 Aug 2018
MPS1-4_S26_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMPS1-4_S26_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70196635
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1789650.25494811823957086No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710120.10116154428200155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA1852050.057.402989
CACCCG2605850.045.1686028
GCACCC2756400.041.004837
GGCACC3086350.034.713396
TGGCAC3318700.030.821015
TTGGCA4338500.022.6445964
CCTTGG5029050.016.5454772
CTTGGC6121800.015.6381593
CGAGAA1632100.014.16320610-14
GCCTTG3452250.013.2221641
ACCCGG1249850.011.4360069
CCGAGA2097050.010.62744710-14
CCCGAG2420300.08.90524310-14
AACCGA639550.08.7045819
GAGAAT2744550.08.64150110-14
AATTCC3193550.08.26722715-19
CAACCG551200.07.94218258
ATTCCA3825300.07.57985815-19
AGAATT3279100.07.549099410-14
GAATTC3062250.07.20367715-19
Produced by FastQC (version 0.11.3)