FastQCFastQC Report
Tue 21 Aug 2018
MPS1-5_S27_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMPS1-5_S27_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84710236
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTCCTTATCTCGTATGC1238750.14623380343315298RNA PCR Primer, Index 18 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG1278350.028.9362741
ACGATC1383800.026.2413834
CGACGA1610050.023.1878932
TCCGAC1125550.016.6494661
GACGAT2505250.015.3276373
CGATCA915050.014.4080685
CGATCC763150.012.514755
ACGTTA319800.011.3180931
CGATCT1147900.09.337065
CGATCG1077950.06.7888275
CGTTAA579800.06.44409372
TAGGTA713100.06.2310225
GGGGGG245837600.05.769833145-146
CGCGAC538400.05.38090751
TAATCG826850.05.36499455
ATTAAC1299150.05.34184551
GTAGGT899650.05.3120254
Produced by FastQC (version 0.11.3)