FastQCFastQC Report
Tue 21 Aug 2018
MPS1-5_S27_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMPS1-5_S27_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84710236
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3255700.3843337185366831No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2095090.24732430210677256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2277150.061.9510239
CACCCG3114250.050.3007558
GCACCC3421450.043.8246737
GGCACC3769700.037.6257746
TGGCAC4024900.033.8007625
TTGGCA5238550.025.1467784
CCTTGG6039350.018.2040822
CTTGGC7256500.017.6610643
CGAGAA2061600.015.00298510-14
GCCTTG4131150.014.4384361
ACCCGG1407550.013.6899079
CCGAGA2598250.011.34341610-14
AACCGA753000.09.8057659
CCCGAG2946600.09.61065210-14
CAACCG651800.09.3133298
GAGAAT3464400.09.11887110-14
AATTCC3982900.08.77086915-19
ACCTTG3194250.08.4218521
ATTCCA4691850.08.20074615-19
AGAATT4030000.08.19046410-14
Produced by FastQC (version 0.11.3)