FastQCFastQC Report
Mon 7 Sep 2015
843.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename843.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97990607
Sequences flagged as poor quality0
Sequence length48
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTA1089650.010.0945161
CGTCTA420750.010.013141
TAGGAC1137350.09.0644354
TTATAC3567900.08.81320643
GTATAG839650.08.5873241
CTTATA4760500.08.35671343
TAGACT935500.08.3289165
CTAGAC909500.08.0540754
CTATAC815400.07.91300734
TAGACG391000.07.90730334
CTACAC1286050.07.88261134
TACACT1116100.07.84034875
GTGTAG1336800.07.47654631
ATACAC2565200.07.40838343
TATACA2948000.07.366271543
TATACT1092300.07.27108145
TTGACG298850.07.121921531
GTGTTA831500.07.0334581
GTCTAG899300.06.9051551
TCTTAT5735150.06.896899742
Produced by FastQC (version 0.11.3)