FastQCFastQC Report
Wed 1 Jul 2015
SRR1703159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1703159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140266028
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA2809580.2003036686830542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCG1760700.027.49335346-47
CGACGA659600.022.86909778-79
CTTATG2889700.022.58867646-47
GTCTCG2402600.022.48313746-47
TATACG1701750.021.935846-47
TACACG1451050.020.57366846-47
CCGACG790250.020.03066876-77
ACGAGT850000.017.29800880-81
CTCTTG4927950.016.29091646-47
TCGGTG967600.016.04745192-93
CTGACG1708850.015.98893194-95
TGCCGA1010900.015.79389674-75
GCGAAT1027550.015.46025638-39
TACCGT402250.014.95782246-47
CGAGTG1049050.014.92409580-81
TGTCGC746750.014.88944546-47
GTCGAC309500.014.11642948-49
CGCTAT375900.013.93520650-51
TCTTAG6016700.013.87065246-47
GGCGAA1109950.013.86131638-39
Produced by FastQC (version 0.11.3)