FastQCFastQC Report
Wed 1 Jul 2015
SRR1930173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25842783
Sequences flagged as poor quality0
Sequence length102
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCG218000.019.69857246-47
CGCTAT61950.016.9823250-51
CGGATA104900.015.43652550-51
TACCGT66350.014.17583548-49
CGCATA56650.014.16369850-51
GTCTCG324800.013.95253646-47
TACACG235550.013.46740946-47
ACCGTA76050.012.463366548-49
GTCGAC47050.011.43802848-49
TGTCGC101050.011.34704248-49
CGTATC48350.011.18039150-51
TATACG263350.011.107120546-47
CTTATG402950.011.06007646-47
CGTCTA313900.010.7463761
CGCTTA66500.010.71702650-51
ACACGT201500.010.6349448-49
CGAGTA110850.010.518650-51
CCGGAT96100.010.26643550-51
GACGTG204000.09.9991157
ACTGCG205350.09.9805698
Produced by FastQC (version 0.11.3)