FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC57610.144025No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG43780.10944999999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAC63750.011.1026544
GTCCTA70650.010.592781
GGACGT28700.010.4905356
AGGACG31300.010.0503265
GACGTG29400.09.7808747
TACGGT4603.9108272E-119.76758414
TCGTCG2905.289635E-69.32670829
CGTCTA11750.08.7982821
TCCGAG20450.08.73052845
GTCGTC10650.08.2298691
GTGTAG51900.08.0975221
TCTCCG25350.08.02363844
CGAACG3403.4093748E-57.95513230
TGTAGG68200.07.9072112
CTCCGA28250.07.839969645
CGTCAA11850.07.77431153
CTCTTA239250.07.74584345
GTAGGA62750.07.66295863
GTATAG38750.07.6556161
TCTCTT325400.07.653689445
Produced by FastQC (version 0.11.3)