FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC51990.12997499999999998No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG42390.10597499999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAC62200.011.6007564
GTCCTA65450.010.9928361
GGACGT28550.010.8633356
GACGTG27100.010.6965927
AGGACG32000.010.1856715
GTCGTC9950.09.0387071
ACTGCG10050.08.9437368
TGTAGG63050.08.8060982
GTGTAG48700.08.4025641
TCCGAG20200.08.27378545
CCTACA67150.08.1010453
GTAGGA64300.08.0406013
TACGCG3952.8496752E-67.9671425
TCTCCG24450.07.944076544
GTATAG38300.07.80768541
TCTCTT322050.07.805409445
CTCTTA233200.07.73823845
CGTCTA11650.07.71975471
CTACAC51600.07.6235984
ACGTGG21500.07.5252188
Produced by FastQC (version 0.11.3)