FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC45350.113375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT26750.011.8618976
GACGTG26650.011.4737137
TAGGAC61750.011.4434494
AGGACG30200.011.2521185
GTCCTA63450.010.1082851
TCCGAG20750.09.23825445
GTATAG39300.08.8757161
TGTAGG65100.08.1236492
CTGTAG73700.07.93907931
ACGTGG23150.07.7697458
TCTCTT327350.07.743602345
TCTCCG26050.07.704107843
GTAGGA61900.07.59836053
CTCTTA236700.07.526930345
CGCGTA4206.4332753E-67.4944727
TTATAC146950.07.36649245
TAGACG13900.07.28554344
CTACAC51050.07.1414034
TACGCG4103.8313876E-57.13548955
TGGCGA42750.07.10020418
Produced by FastQC (version 0.11.3)