FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC44260.11065000000000001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAC60700.010.1978744
GTCCTA63500.09.8906151
CGACGA3505.3785698E-89.63763823
GGACGT27300.09.4820196
CGTCTA11700.09.4276421
AGGACG31400.09.247535
GACGTG28350.08.9623937
GATCGG3352.912926E-58.0636061
TGTAGG65050.07.9934782
CTCTTA207950.07.89147445
GTATAG39600.07.7310161
TCTCTT292100.07.61677745
TTATAC130100.07.58896445
TAGACG13350.07.5874694
GTGTAG51400.07.53283071
CTACAC50500.07.3545864
CCGACG4308.76247E-67.32161722
GCTCGA6809.931682E-107.27510911
CTATAC40350.07.25212864
CGATAG4053.3260225E-57.217862610
Produced by FastQC (version 0.11.3)