FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC56180.14045No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG43460.10865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG3655.9117156E-1211.70194422
CGACGA2954.409685E-911.43053323
GACGTG26550.011.00731857
GGACGT27950.010.8594786
GTCCTA67850.010.6787771
TAGGAC62150.010.6356294
AGGACG32000.010.2579335
TCCGAG21100.08.55757945
GTGTAG49100.08.5240691
TGTAGG65400.08.49282552
GTATAG41450.07.92581271
TCCTAC61600.07.7025542
CCTACA68750.07.62107473
CTCTTA239250.07.55655745
CTACAC48400.07.5253494
CGTGAT18950.07.486492628
ACTGCG9050.07.4520438
TACGAC8800.07.40921744
GTAGGA65250.07.3750723
TTATAC140350.07.188503344
Produced by FastQC (version 0.11.3)