FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_006.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_006.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC49310.123275No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG40110.10027499999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAC64800.010.8243734
GGACGT28000.010.7589516
TACGCG4258.6402E-1210.5794785
AGGACG32400.010.40805155
GACGTG28350.010.2287327
GTCCTA66800.010.0763841
CGCGTA4705.820766E-119.5657127
CTATAC39900.08.958754
ACGCGT5102.728484E-108.8162326
TGTAGG64600.08.5268642
GTATAG38950.08.3805111
CGTCTA13550.08.1407941
GTAGGA62300.08.1198913
ACGTGG22900.07.8530748
ACTGCG9750.07.83897838
CGTCGT3751.2549333E-57.808289528
TCTCCG20850.07.794442744
TCCGAG17500.07.738768645
CTGTAG68450.07.6299991
TCTCTT320800.07.57070945
Produced by FastQC (version 0.11.3)