FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_007.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_007.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC43320.1083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT27750.011.9928656
GACGTG27500.011.8542117
AGGACG31500.011.8509825
TAGGAC60650.011.16072754
GTCCTA67650.010.118391
CCTCGC21750.08.09351645
TCCGAG20650.07.978195745
CGCGTA4003.364124E-67.86874347
CGTCTA12600.07.8629911
CGAACG3151.328368E-47.862203630
ACCGTC6302.1282176E-107.85089978
TGTAGG64000.07.80072932
TCCTCG25250.07.776062544
TACGCG4053.955643E-67.77373655
TCTCTT308250.07.760767545
CTCTTA226650.07.73690345
TAGACG13550.07.6344074
ACGTGG23250.07.542398
ACTGCG10450.07.52989778
GTAGGA63450.07.51391843
Produced by FastQC (version 0.11.3)