FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC47930.11982500000000001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACG30800.011.2486035
TAGGAC64200.011.0733914
GACGTG28500.010.81228357
GGACGT28400.010.7720986
CGACGA3504.878075E-910.28263823
GTCCTA69400.09.6162691
TAGACG12950.09.03362754
TACGCG4208.0580776E-88.5703645
CGCGTA3953.0794718E-78.5415337
CGAACG3302.451867E-58.18387130
TGTAGG65950.08.1528922
GTATAG38100.07.98883061
TCCGAG17900.07.92494745
CCGACG4603.210439E-77.823745322
CTCTTA207300.07.820635345
GTCGTC10500.07.7301441
ACGCGT4704.4343142E-77.6577616
CGATAG4806.089258E-77.497756510
GTACTA29550.07.47726871
TCTCCG22200.07.4042144
Produced by FastQC (version 0.11.3)