FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC50770.126925No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG40800.10200000000000001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAC61750.011.43944554
GTCCTA66200.010.425211
GGACGT26400.010.3088996
AGGACG29100.010.2818425
GACGTG26900.09.2811457
CGAACG3252.0916464E-69.005330
TCCGAG21050.08.76430545
TACGCG4603.6148776E-88.3138725
CGCGTA4105.254601E-78.2288327
TCTCCG24700.08.19787544
CGACGA3607.664332E-68.12277223
CTATAC39050.08.0076684
TGTAGG65550.07.99641852
TCCTCG23750.07.76794244
CTCTTA226000.07.745097645
CCTACA71300.07.60395963
TCCTAC64700.07.54514742
TCTCTT311000.07.52365245
CCTCGC23450.07.483546345
CGTCTA11800.07.45425031
Produced by FastQC (version 0.11.3)