FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_010.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_010.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC48680.1217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT27700.010.8790676
GTCCTA64150.010.6899521
CGCGTA3851.8098945E-1010.5142537
GACGTG29050.010.3734997
TAGGAC65050.010.2690454
TACGCG3801.6275408E-910.0620455
AGGACG32450.09.9808775
TGTAGG68400.08.3192722
CGACGA3801.6866493E-68.28525123
CGTCTA11600.08.1675181
TCTCCG25450.08.13718244
CTCCGA26400.08.10016245
ACGTGG24550.07.9688638
GTGTAG51450.07.8919791
GTATAG37150.07.8330291
CTCTTA232650.07.750031545
TCTCTT326500.07.68026145
ACGCGT5051.6152399E-77.5704936
GTAGGA66000.07.3949753
GTGCGA7357.9580786E-117.35616227
Produced by FastQC (version 0.11.3)