FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC41270.10317499999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT28000.010.6866386
TAGGAC61050.010.2460274
GACGTG27100.010.2113547
GTCCTA65250.010.1866311
AGGACG31800.09.6937065
TCCGAG20450.09.69337745
TACGCG4401.7603725E-88.69345955
GCTCGA7350.08.5679511
GATCGG3801.6440172E-68.301061
ACGTGG20550.08.207738
TGTAGG65000.08.0656422
TCTCCG24100.07.851415643
GTATAG40300.07.7713931
CGACGA2905.194352E-47.75304923
TAGACG13400.07.72412734
GTGTAG52550.07.67484141
CTCTTA218500.07.577424545
TCTCTT304000.07.52845545
GTAGGA63500.07.44118263
TTTCCG26650.07.3393924
Produced by FastQC (version 0.11.3)