FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC59300.14825000000000002No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG46650.11662499999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGA2751.5556907E-912.26339323
GGACGT27450.012.1226796
CCGACG2852.6425369E-911.83309922
AGGACG31450.011.5821695
GACGTG27700.011.2820277
GTCCTA62700.010.996841
TAGGAC62800.010.8845244
TCCGAG21550.08.88673345
CGCGTA3801.7753428E-78.8748257
GTATAG37500.08.6525731
AAGTCG10650.08.4442777
GGTCGG9350.08.41605411
GTCGTC10900.08.2506019
ACTGCG8850.08.1294058
TACGCG4301.0406866E-67.8436525
TCTCTT322150.07.77709145
TGTAGG63900.07.74136162
ACGTGG23150.07.5752288
TACAGT68000.07.57220035
CCTACA70500.07.5588333
Produced by FastQC (version 0.11.3)